SPHK1 anticorps (Internal Region)

N° du produit ABIN308402

L’anticorps Chèvre Polyclonal anti-SPHK1 a été validé pour WB, ELISA et IP. Il convient pour détecter SPHK1 dans des échantillons de Humain.

Aperçu rapide pour SPHK1 anticorps (Internal Region) (ABIN308402)

Antigène: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SPHK1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Classe de qualité: Verified

Détail du produit anti-SPHK1 anticorps

Épitope: Internal Region

Fonction: SPHK1

Séquence: DVDLESEKYR RLGE

Specificité: This antibody is expected to recognise all three reported isoforms (NP_068807.2, NP_892010.2, NP_001136073.1). Reported variants represent identical protein (NP_001136073.1, NP_001136074.1).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-DVDLESEKYRRLGE, from the internal region of the protein sequence according to NP_068807.2, NP_892010.2, NP_001136073.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunoprecipitation: EB05266 has been used in nuclear lysates of HeLa cells and a band of ~45kDa band has been detected.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur SPHK1

Antigène: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Autre désignation: SPHK1

Sujet: SPHK1, sphingosine kinase 1, SPHK, SK1, SKase

ID gène: 8877, 20698, 170897

NCBI Accession: NP_068807, NP_892010, NP_001136073

Pathways: VEGF Signaling